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. 2021 Jan 25;41(2):e00445-20. doi: 10.1128/MCB.00445-20

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Copyright © 2021 Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — miR-149 reversed the effects of circRNA 100146 on proliferation, apoptosis, migration, and invasion of CRC cells. (A and B) The viability of SW620 (A) and SW480 (B) cells transfected with sh-NC, sh-circRNA 100146 no. 1, sh-NC plus anti-miR-149, or sh-circRNA 100146 no. 1 plus anti-miR-149 was examined by MTT assay. (C) The apoptosis of transfected SW620 and SW480 cells was evaluated by flow cytometry assay. (D and E) The migration and invasion of transfected SW620 (D) and SW480 (E) cells were assessed by transwell assay. (F and G) Western blot assay was conducted to detect the expression of proteins (PCNA, Bcl-2, E-cad, and MMP9) in transfected SW620 (F) and SW480 (G) cells. *, P < 0.05.