Figure 2.

LNPs with T7 pass through the transwell cell barrier and are taken up by target cells. HeLa (CCR5-negative control cell) or TZM-bl (CCR5-positive cell type) cells (A and B) as well as HEK293T or gp160 positive HEK293T cells (C and D) were seeded at a density of 50,000 cells/well. The next day, transwell inserts containing confluent hMEC/D3 cells at trans-endothelial electrical resistance (TEER) above 30 Ω⋅cm² were placed into experimental wells, LNPs (1000:1) were added to the apical surface, and 24 h later, the target cells were processed for Cy5 detection using fluorescence-activated single-cell sorting (FACS). A) Percentage of cells positive for Cy5 detection in HeLa and TZM-bls. B) MFI of Cy5 in each cell population in HeLa and TZM-bls. C) Percentage of cells positive for Cy5 detection in HEK cell types. D) MFI of Cy5 in each cell population in HEK cell types. Histograms are representative of three independent biological experiments, each containing duplicate technical replicates.