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. 2021 Mar 23;20:1533033821990036. doi: 10.1177/1533033821990036

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — miR-301b-3p targets and inhibits DLC1 expression (A) Volcano plot of DEmRNAs in normal group and tumor group in TCGA-LUAD dataset. Red dots refer to differentially up-regulated genes and green dots refer to differentially down-regulated genes; (B) Venn diagram of predicted target genes of miR-301b-3p on TargetScan and miRDB database and down-regulated DEmRNAs; (C) Pearson correlation analysis of miR-301b-3p and 9 candidate target genes; (D) Box plots of DLC1 expression in normal group and tumor group in TCGA-LUAD; (E) The expression level of DLC1 mRNA in human normal lung epithelial cell lines (BEAS-2B, 16HBE) and LUAD cell lines (H1975, HCC78, PC9, SPCA1) was detected by qRT-PCR; (F) The binding site sequence of miR-301b-3p on DLC1 3’UTR was predicted by TargetScan database and was verified by dual-luciferase assay; Relative expression of (G) DLC1 mRNA and (H) DLC1 protein after overexpressing miR-301b-3p were assessed by qRT-PCR and Western blot, respectively; *p < 0.05.