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. 2020 Jun 8;8(3):353–363. doi: 10.1016/j.gendis.2020.05.007

Figure 2.

Fig. 2

Expression pattern of THBS1 in BeWo cell fusion model. (A) immunofluorescence detection of ECAD (green) and THBS1 (red) in BeWo cells treated with 25 μm FSK for 72 h. Nuclei are stained with DAPI (blue). Arrows indicate fused BeWo cells. Scale 50 μm; (B) Western blot analysis of the expression of THBS1 and the syncytialization markers (ECAD, GCM1, Syn2). β-actin was used as a loading control (a). The quantification of Western blot results was normalized to β-actin (b); (C) qRT-PCR detection of the mRNA of THBS1, CD36 and syncytialization markers in the BeWo cell fusion model. Results are expressed as: mean ± SD relative to control (1.0). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.