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. 2021 Apr 20;9:632779. doi: 10.3389/fcell.2021.632779

FIGURE 2.

FIGURE 2

IATL and cisplatin combination triggers ROS-dependent apoptosis in prostate cancer cells. (A,B) DU145 or PC-3 cells were treated with IATL or cisplatin alone or their combination at the indicated concentration for 2 h; cellular ROS levels were detected by using the DCFH-DA probe. (C,D) DU145 or PC-3 cells were preincubated with NAC (5 mM) for 2 h before exposure to the combination of IATL and cisplatin for 2 h; cellular ROS levels were detected by using the DCFH-DA probe. (E,F) DU145 or PC-3 cells were preincubated with NAC (5 mM) for 2 h; cell viability was detected by an MTT assay after being treated with the combination of IATL and cisplatin for 24 h. (G,H) DU145 or PC-3 cells were preincubated with catalase (2,000 U/mL) for 2 h; cell viability was detected by an MTT assay after being treated with the combination of IATL and cisplatin for 24 h. (I–L) DU145 or PC-3 cells were preincubated with NAC (5 mM) for 2 h; the cell apoptosis rate was detected by flow cytometry after being treated with the combination of IATL and cisplatin for 24 h. (M,N) DU145 or PC-3 cells were preincubated with NAC (5 mM) for 2 h; caspase-3 and caspase-9 activity was measured by an assay kit after being treated with the combination of IATL and cisplatin for 20 h. *p < 0.05, **p < 0.01.