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. 2021 Apr 20;12:667474. doi: 10.3389/fphar.2021.667474

FIGURE 1.

FIGURE 1

Up-regulation of VEGFR-3 in human prostate cancer PC-3 cells. The protein expression of VEGFRs was examined in human prostate epithelial PrEC cells and prostate cancer LNCaP (androgen-dependent/weakly metastatic), PC-3 (androgen-independent/highly bone metastatic), and DU145 (androgen-independent/moderate brain metastatic) cells by Western blotting. (A) Representative blots of VEGFR-1, VEGFR-2, and VEGFR-3 in PrEC, LNCaP, PC-3, and DU145 cells. β-actin was used as an endogenous marker. (B) Expression levels of VEGFR-1, VEGFR-2, and VEGFR-3 in PrEC, LNCaP, PC-3, and DU145 cells (n = 4–11). The expression levels of VEGFRs were normalized by that of β-actin. Note that the expression level of VEGFR-3 was higher in PC-3 cells than in PrEC, LNCaP, and DU145 cells. Data are presented as means ± S.D.