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. 2021 Apr 20;12:667474. doi: 10.3389/fphar.2021.667474

FIGURE 2.

FIGURE 2

Abundant expression of VEGFR-3 in PC-3 cells. The expression of VEGFR-3 protein was examined in PrEC, LNCaP, PC-3, and DU145 cells by Western blotting using three different antibodies for VEGFR-3. (A) Representative blots of VEGFR-3 in PrEC, LNCaP, PC-3, and DU145 cells using three different VEGFR-3 antibodies: #79-633 (ProSci), sc-28297, and sc-321 (Santa Cruz Biotechnology). (B) Expression level of VEGFR-3 in PrEC, LNCaP, PC-3, and DU145 cells (n = 4). The expression level of VEGFR-3 were normalized by that of β-actin. (C) Representative blots of VEGFR-3 in PC-3 cells treated with 50 nM control or VEGFR-3 siRNA for 48 h. (D) Expression level of VEGFR-3 in PC-3 cells treated with control or VEGFR-3 siRNA (n = 6). Data are presented as means ± S.D. **p < 0.01 vs. control siRNA (unpaired two-tailed t-test).