FIGURE 1.

(A) Schematic overview of cell synchronization by centrifugal elutriation. The inlet tube is controlled by a three-way valve, which allows the continuous loading of media and/or cells. The bubble trap is half filled with growth medium and the remaining air cushion acts as a damper of the pulsatile flow from the pump. The manometer is placed downstream of the bubble trap. The outlet tube is connected to a collection flask during elutriation. (B) Principle of the counter-flow centrifugal elutriation. Cells are separated on the basis of size and density by gradually changing the balance of inward fluid velocity termed “counter-flow” (driving cells toward the axis of rotation) and outward centrifugal force (driving cells away from the axis of rotation).