TABLE 1.

Factors affecting flow cytometry.

Factors	Options	Effects	Nuclear adhesion ratio (%)
Number of cells	>2 × 106 cells (1 × 107 cells)	Inadequate lysis and severe nuclei adhesion	51.47
	= 2 × 106 cells	Minimal lysis and/or nuclear adhesion	9.00*
	<2 × 106 cells (1 × 105 cells)	Nuclei scarcity	N/A
Centrifugal force for nuclei	>130 g (800 g)	Severe nuclei adhesion	63.01
	= 130 g	Minimal lysis and/or nuclear adhesion	9.00*
	<130 g (50 g)	Severe nuclei loss	N/A
Rotor type	Fixed-angle rotor	Severe nuclei adhesion	43.61
	Swing rotor	Good	9.00*
Vortex	−	Inadequate lysis or ineffective separation of macronucleus (MAC) from micronucleus (MIC)	16.75
	+	Good lysis and nuclear separation	9.00*
Nuclei wash buffer	−	Severe nuclei adhesion	65.41
	+	Good	9.00*
1% NP-40	−	Slight nuclei adhesion	9.69
	+	No detectable nuclear adhesion	9.00*
70% Ethanol	−	Severe nuclei adhesion or propidium iodide (PI)/RNase staining buffer cannot enter the nucleus	26.77
	+	No nuclear adhesion, strong PI signals	9.00*

*Optimized condition for MAC purification.