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. 2021 Apr 20;9:619444. doi: 10.3389/fcell.2021.619444

FIGURE 1.

FIGURE 1

Conditional medium derived from VSMCs stimulated with PDGF induces miR-185 expression in ECs and attenuates angiogenesis. (A) Levels of miR-185 in the supernatant of VSMCs under basal conditions (Basal) or exposed to PDGF-BB (20 ng/ml) (PDGF) for 24 h. (B) Levels of miR-185 in ECs exposed to different media for 24 h were determined by qRT-PCR assays. Basal, basal medium; PDGF, PDGF treatment (20 ng/ml); Basal-CM, medium from VSMCs in basal conditions; PDGF-CM, medium from VSMCs after PDGF stimulation. (C) Proliferation of ECs exposed to different media for 24 h as assessed by a BrdU assay. (D) Migration of ECs exposed to different media for 24 h assessed by a scratch wound assay. Migration is expressed as the relative recovery rate of the scratch wound. (E) Angiogenesis of ECs exposed to different media for 24 h was assessed by a tube formation assay. The quantitative analysis of the tube lengths is shown. *P < 0.05 vs. Basal, #P < 0.05 vs. Basal-CM. (F–I) ECs were exposed to media from SMCVs stimulated with PDGF transfected with miR-185 inhibitor or its negative control for 24 h. Levels of miR-185 in ECs (F), cell proliferation (G), cell migration (H), and tube formation (I) of ECs. *P < 0.05.