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. 2021 Apr 20;9:619444. doi: 10.3389/fcell.2021.619444

FIGURE 2.

FIGURE 2

miR-185 was transferred from PDGF-stimulated VSMCs to ECs via exosomes. (A) Representative electron microscopy image of isolated exosomes from the supernatant of VSMCs under both basal (Basal-Exo) and PDGF conditions (PDGF-Exo). (B) Representative NTA histogram of isolated exosomes from the supernatant of VSMCs under both basal (Basal-Exo) and PDGF conditions (PDGF-Exo). (C) Western blot analysis of exosome proteins. (D) qRT-PCR detection of miR-185 expression in exosomes derived from VSMCs in basal condition and from VSMCs exposed to 20 ng/mL PDGF for 24 h. *P < 0.05. (E) Levels of miR-185 in ECs incubated with CM from VSMCs in basal conditions and from VSMCs exposed to 20 ng/mL PDGF were treated with or without RNase I and/or Triton X-100. *P < 0.05 vs. Basal-CM, #P < 0.05 vs. RNAse-PDGF-CM. (F) Levels of miR-185 in ECs exposed to different media or exosomes derived from VSMCs following different treatment. *P < 0.05 vs. Basal-CM or Basal-Exo, #P < 0.05 vs. PDGF-Exo. (G) Internalization of PKH67-labeled exosomes derived from VSMCs transfected with Cy3-miR-185.