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. 2021 Apr 20;9:619444. doi: 10.3389/fcell.2021.619444

FIGURE 5.

FIGURE 5

Exosomal miR-185 transfer from VSMCs to ECs is modulated by hnRNPA2B1. (A) Western blot analysis of hnRNPA2B1 in VSMCs in basal condition and from VSMCs exposed to 20 ng/mL PDGF for 24 h and their exosomes. A2B1: hnRNPA2B1; s-A2B1: sumoylated hnRNPA2B1. *P < 0.05, **P < 0.01. (B) Expression of hnRNPA2B1 in VSMCs transfected with control shRNA (sh-Ctrl) or hnRNPA2B1 shRNA (sh-hnRNPA2B1) carrying lentivirus for 24 h and then stimulated with PDGF for 24 h. *P < 0.05, **P < 0.01 vs. control group, #P < 0.05 vs. PDGF group. (C) Expression of miR-185 in exosomes derived from VSMCs transduced with control shRNA (sh-Ctrl) or hnRNPA2B1 shRNA (sh-hnRNPA2B1) carrying lentivirus for 24 h and then stimulated with or without PDGF for 24 h. *P < 0.05 vs. control group, #P < 0.05 vs. PDGF group. (D) Proliferation of ECs treated with exosomes derived from VSMCs transduced with control shRNA (sh-Ctrl) or hnRNPA2B1 shRNA (sh-hnRNPA2B1) carrying lentivirus for 24 h and then stimulated with or without PDGF was assessed by a BrdU assay. (E) Migration of ECs treated with exosomes derived from VSMCs transfected with control shRNA (sh-Ctrl) or hnRNPA2B1 shRNA (sh-hnRNPA2B1) carrying lentivirus for 24 h and then stimulated with or without PDGF was assessed by a scratch wound assay. Quantification of migration is expressed as the relative rate of scratch wound healing. (F) Angiogenesis of ECs treated with exosomes derived from VSMCs transfected with control shRNA (sh-Ctrl) or hnRNPA2B1 shRNA (sh-hnRNPA2B1) carrying lentivirus for 24 h and then stimulated with or without PDGF was assessed by a tube formation assay. Quantitative analysis of the tube lengths is shown. *P < 0.05 vs. Basal group, #P < 0.05 vs. PDGF-Exo group.