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. 2021 Apr 20;9:672267. doi: 10.3389/fchem.2021.672267

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Copyright © 2021 Cecchini, Pannilunghi, Tardy and Scapozza.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic representation of the NanoBret assay. The NanoLuc (nLuc) luciferase-tagged protein interacts with a fluorescent tracer to produce a bioluminescent resonance energy transfer (BRET). The addition of PROTACs to cells results in a competition between the degrader molecule and the tracer in protein binding. The recorded BRET signal decreases accordingly to PROTAC efficiency in target engagement.