FIGURE 2.

CRISPR-Cas9 component formats and advances to enhance editing efficiency. (A) CRISPR-Cas9 consists of a Cas9 nuclease and a gRNA that can be used in different formats (plasmid, mRNA, or protein) to form the RNP complex. (B) A DNA donor can also be used to generate KI models, also in different formats (ssODN, lsDNA, plasmid, dsDNA). In red are indicated advances to enhance efficacies of editing. Other Cas used for rodent models generation are described in Table 3. Figure created with BioRender.com. IVT, in vitro transcribed; RNP, ribonucleoprotein complex; DSB, double-strand break; ssODN, single-stranded oligonucleotide; lsDNA, long single-stranded DNA; dsDNA, linear double-stranded DNA.