FIGURE 2.

Effects of CRNDE on M2 polarization of THP‐1 cells and the mechanism. A‐E, Detection of M2 macrophage marker expression by CRNDE (n = 5): The expression of M2 macrophage markers in the THP‐1‐pcDNA3.1 group was significantly increased, which was significantly different from the THP‐1‐Con group. Meanwhile, the expression of M2 macrophage markers in THP‐1‐shRNA group was lower than that of the THP‐1‐Con group. Comparison between groups, *P <.05; **P <.01. F‐I, Effects of CRNDE on JAK‐STAT6 and AKT1 signal expression (n = 3): The expression of JAK1, STAT6, p‐STAT6, AKT1 and p‐AKT1 in the THP‐1‐pcDNA3.1 group was significantly higher than that of the THP‐1‐Con group. Meanwhile, the expression level in the THP‐1‐shRNA group was lower than that of the THP‐1‐Con group. Comparison between groups, *P <.05; **P <.01