FIGURE 3.

Effects of CRNDE on M2 polarization of THP‐1 induced by liver cancer cells H22. A, Cell viability results (n = 5): The cell viability of the THP‐1‐pcDNA3.1 group was significantly higher than that of the THP‐1‐Con group. The expression of CRNDE was associated with THP‐1 cell viability. Comparison with THP‐1‐Con, *P <.05; **P <.01. B and C, Proportion of F4/80 + CD163+M2 macrophages by flow cytometry (n = 5): The proportion of F4/80 + CD163+M2 macrophages in the THP‐1‐pcDNA3.1 cells was significantly higher than that of THP‐1‐Con group, while the proportion in the THP‐1‐shRNA group was significantly lower than that of THP‐1‐Con group. Comparison between groups, *P <.05; **P <.01. D, CD163 expression by immunofluorescence staining (n = 3): The expression of CD163 in the THP‐1‐pcDNA3.1 group was significantly higher than that in the THP‐1‐Con group, while the expression of CD163 in the THP‐1‐shRNA group was lower than that of the THP‐1‐Con group