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. 2021 Apr 1;25(9):4455–4465. doi: 10.1111/jcmm.16515

FIGURE 4.

FIGURE 4

ExoDiff, and not ExoPr0, delays mPTP opening in cells via gp130 and JAK1/2. HL‐1 cells were treated with various treatments and inhibitors for 15 min prior to assessing the time until mPTP opening using the assay described in Figure 3. (A) HL‐1 cells were pre‐incubated with vehicle (DMSO), ExoDiff, ExoPr0, or 0.2 μmol/L cyclosporin A (CsA), (n = 6,6,3,3,3,2 in each group from left to right). (B) PI3K was inhibited with 15 μmol/L PI3K inhibitor LY294002 (LY), (n = 3,3,2,2,3). (C) MAPK was inhibited with 50 μmol/L MAPK inhibitor PD98059 (PD), (n = 3 in all groups). (D) TLR4 was inhibited with 5 μmol/L TAK242 (n = 5,6,5,4,4). (E) JAK1/2 was inhibited with 0.1 μmol/L Ruxolitinib (Rux), n= (3,3,4,4,2). (F) gp130 receptor was inhibited with 1 μmol/L SC144 (n = 7,6,5,5,5). Mean ±SEM is plotted. *P <.05; **P <.01; ***P <.001, as indicated