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Immunofluorescence and siRNA knockdown of PNN in renal cell carcinoma cell lines. A, Immunofluorescence assay to detect PNN in Caki‐1 cell lines. B, Transfection efficiency of siRNA detected by qRT‐PCR and Western blot analyses. Data are presented as the mean ±standard deviation (SD) calculated from three experiments. *, P <.05
