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. 2021 Mar 30;25(9):4373–4386. doi: 10.1111/jcmm.16500

FIGURE 1.

FIGURE 1

Screening of miRNAs directly targeting NLRP3. A, Venn diagram of four independent miRNA differential analysis databases reveals 21 possible miRNAs directly targeting NLRP3. B, qRT‐PCR analysis of miR‐302c‐3p levels in healthy arterial tissues (n = 10) and atherosclerotic arterial samples (n = 14). C, qRT‐PCR analysis of NLRP3 levels in healthy arterial tissues (n = 7) and atherosclerotic arterial tissues (n = 12). D, miR‐302c‐3p expression in human normal and atherosclerotic arteries, as detected by FISH. E, qRT‐PCR analysis of miR‐302c‐3p expression in HUVECs treated with 100 µg/mL ox‐LDL at the indicated times (0, 6, 12 and 24 h). F, qRT‐PCR analysis of NLRP3, caspase‐1, and IL‐1β expression in HUVECs treated with 100 µg/mL ox‐LDL at the indicated times (0, 6, 12 and 24 h). Scale bars: 50 μm. *P < .05; **P < .01; ***P < .001. Error bars indicate the mean ± SEM of at least triplicate independent experiments