Skip to main content
. 2021 Mar 30;25(9):4373–4386. doi: 10.1111/jcmm.16500

FIGURE 2.

FIGURE 2

MiR‐302c‐3p directly targets and inhibits NLRP3 expression. A, Predicted miR‐302c‐3p target sequences in the NLRP3 3′ UTR of different species. B, The transfection efficiency of miR‐302c‐3p is shown, as detected by qRT‐PCR. C‐E, qRT‐PCR and Western blot analysis of NLRP3 expression in HUVECs transfected with the miR‐302c‐3p mimic, miR‐302c‐3p mimic negative control (NC), miR‐302c‐3p inhibitor and miR‐302c‐3p inhibitor NC (in‐NC). F, Expression levels of NLRP3 are shown, as tested by qRT‐PCR after biotinylated miR‐302c‐3p or its mutant were transfected into HUVECs for 24 h. G, Luciferase activity in 293T cells cotransfected with the miR‐302c‐3p mimic or miR‐302c‐3p NC and NLRP3 3′ UTR wild‐type (WT) or mutant (Mut) recombinant plasmids containing the miR‐302c‐3p binding sites. *P < .05; **P < .01; ***P < .001. Error bars indicate the mean ± SEM of at least triplicate independent experiments