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. 2021 Mar 30;25(9):4373–4386. doi: 10.1111/jcmm.16500

FIGURE 3.

FIGURE 3

Overexpression of miR‐302c‐3p reverses ox‐LDL‐induced pyroptosis in HUVECs. HUVECs were transfected with the NC or miR‐302c‐3p mimic and then treated with 100 µg/mL ox‐LDL. A, qRT‐PCR analysis of NLRP3, caspase‐1 and IL‐1β expression. B and C, Western blot analysis and quantification of NLRP3, GSDMD, casp1 p20 and IL‐1β expression. D, Lactate dehydrogenase (LDH) release assay detecting LDH activity in the cell supernatant. E, Representative fluorescence micrographs of cells stained with propidium iodide (PI, red) and Hoechst 33 342 (blue). F, Quantification of the percentage of PI‐positive cells. PI‐positive cells are reduced in the miR‐302c‐3p mimic + ox‐LDL group compared to those in the NC + ox‐LDL group. Scale bars: 50 μm. *P <.05; **P <.01; ***P <.001. Error bars indicate the mean ± SEM of at least triplicate independent experiments