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. 2021 Mar 30;25(9):4373–4386. doi: 10.1111/jcmm.16500

FIGURE 5.

FIGURE 5

Silencing of NLRP3 expression diminishes the miR‐302c‐3p effect on pyroptosis. A, qRT‐PCR analysis of NLRP3 expression in HUVECs transfected with si‐NC, si‐NLRP3 1 and si‐NLRP3 2, respectively. B and C, Western blot analysis and quantification of NLRP3 expression in HUVECs transfected with si‐NC, si‐NLRP3 1 and si‐NLRP3 2, respectively. D, qRT‐PCR analysis of NLRP3 expression in HUVECs transfected as indicated and untreated with ox‐LDL. E‐H, qRT‐PCR analysis of NLRP3, caspase‐1, GSDMD and IL‐1β expression in HUVECs transfected as indicated and then treated with 100 µg/mL ox‐LDL. I, Western blot analysis of NLRP3, GSDMD, casp1 p20 and IL‐1β expression in HUVECs treated as indicated. J, Lactate dehydrogenase (LDH) release assay detecting LDH activity in the cell supernatant of HUVECs treated as indicated. K, Representative fluorescence micrographs of cells stained with propidium iodide (PI, red) and Hoechst 33 342 (blue). Scale bars: 50 μm. *P < .05; **P < .01; ***P < .001. Error bars indicate the mean ± SEM of at least triplicate independent experiments