FIGURE 4.

Overexpression of CDR1as up‐regulates the expression of stemness markers and PDLSC osteogenic differentiation. A. Approximately 80% of PDLSCs were red fluorescent, and thus successfully transfected by lentivirus, in the ov‐NC and ov‐CDR1as groups. The expression levels of CDR1as in these groups were analysed by qRT‐PCR. B. mRNA and protein expression levels of stemness‐associated genes (SOX2, OCT4 and Nanog) as measured by qRT‐PCR and Western blot in the ov‐CDR1as and ov‐NC groups. C. Mineralized matrix deposition by PDLSCs cultured with osteogenic inductive medium for 21 days, as demonstrated by Alizarin Red staining and quantified by CPC assay. D. Protein expression levels of ALP and Runx2 after culturing with osteogenic inductive medium for 7 days, as demonstrated by ALP staining and Western blot. E. Oil deposition of PDLSCs after culturing with adipogenic inductive medium for 21 days, as demonstrated by Oil Red O staining and quantified by optical absorbance at 510 nm after adding isopropyl alcohol. Scale bar (A, C, D, E), 100 μm. Quantitative qRT‐PCR data are presented as mean ± SD of three experiments. *P < .05; **P < .01; NS, not significant, by Student's t test