FIGURE 6.

hnRNPM promotes the expression of CDR1as in PDLSCs. In the RNA pull‐down assay, the biotinylated CDR1as probe (biotin‐CDR1as) was designed to pull down CDR1as and RBPs. Biotinylated random oligo (biotin‐NC) was used as a negative control. A. Normalized CDR1as levels in input and biotin‐CDR1as and biotin‐NC elutions of the pull‐down assay, as measured by qRT‐PCR. B. Silver staining of eluted samples from the PDLSC pull‐down assay. C. hnRNPM protein levels in eluted samples from the pull‐down assay, as measured by Western blot. D. hnRNPM mRNA expression levels in PDLSCs transfected with si‐NC and four siRNAs targeting different regions of hnRNPM, as analysed by qRT‐PCR. E. hnRNPM protein expression levels in PDLSCs transfected with si‐NC and four siRNAs targeting different regions of hnRNPM, as analysed by Western blot. F. CDR1as levels in PDLSCs transfected with si‐NC and four siRNAs targeting different regions of hnRNPM, as analysed by qRT‐PCR. Quantitative qRT‐PCR data are presented as mean ± SD of three experiments. **P < .01, by Student's t test