FIGURE 7.

CDR1as regulates stemness of PDLSCs via miR‐7 and KLF4. A. The expression levels of miR‐7 and KLF4 in the sh‐CDR1as#1 and sh‐NC groups were analysed. B. Schematic illustration showing the differences between the two luciferase reporters, including one containing the complete KLF4 3′‐UTR sequence (KLF4‐wt) and one containing the KLF4 3′‐UTR sequence, with mutated sequences in the two miR‐7 binding sites (KLF4‐mut). The reporter assay showed the luciferase activity of KLF4‐wt and KLF4‐mut in 293T cells co‐transfected with miR‐7 mimics. C. KLF4 mRNA and protein expression levels in PDLSCs transfected with si‐NC and four siRNAs targeting different regions of KLF4, as analysed by qRT‐PCR and Western blot. D. mRNA and protein expression levels of stemness‐associated genes (SOX2, OCT4 and Nanog) in PDLSCs transfected with si‐NC and siRNA targeting KLF4, as measured by qRT‐PCR and Western blot. Quantitative qRT‐PCR data are presented as mean ± SD of three experiments. **P < .01, by Student's t test