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. 2021 May 4;11(5):e403. doi: 10.1002/ctm2.403

FIGURE 2.

FIGURE 2

COMMD10 inhibits TNFα mediated ubiquitination and degradation of IκBα and suppresses the NF‐κB signaling pathway. (A) Effect of COMMD10 deficiency on the level of IκBα, p‐IκBα in HepG2/Vector and HepG2/siCOMMD10 cells exposed to TNFα (10 ng/ml) as illustrated. Line graph showed the gray scale value of IκBα at indicated time in HepG2/Vector and HepG2/siCOMMD10 cells. (B) Effect of COMMD10 overexpression on the level of IκBα, p‐IκBα in SMMC7721/Mock and SMMC7721/COMMD10 cells exposed to TNFα (10 ng/ml) as illustrated. Line graph showed the gray scale value of IκBα at indicated time in SMMC7721/Mock and SMMC7721/COMMD10 cells. (C) Effect of COMMD10 deficiency on the level of IκBα, p‐IκBα in HepG2/Vector and HepG2/siCOMMD10 cells exposed to MG132 (40μM) and TNFα (10 ng/ml) as illustrated. Line graph showed the gray scale value of IκBα at indicated time in HepG2/Vector and HepG2/siCOMMD10 cells. (D) Effect of COMMD10 overexpression on the level of IκBα, p‐IκBα in SMMC7721/Mock and SMMC7721/COMMD10 cells exposed to MG132 (40μM) and TNFα (10 ng/ml) as illustrated. Line graph showed the gray scale value of IκBα at indicated time in SMMC7721/Mock and SMMC7721/COMMD10 cells. (E) Effect of COMMD10 deficiency on the level of IκBα ubiquitination in HepG2/Vector and HepG2/siCOMMD10 cells exposed to TNFα (10 ng/ml)