FIGURE 4.

COMMD10/NF‐κB/Bcl‐2/Bax/Caspase9/3 axis associates with apoptosis. (A) Annexin V‐fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining of indicated cells treated with irradiation (6 Gy). (B) A series of apoptosis assay induced by cisplatin (10ug/ml). Representative images of AOEB staining (upper), TUNEL assay (middle) and caspase3/7 activation assay (bottom) were showed from top to bottom, and the corresponding statistical analysis chart arranged from left to right. (C) Western blot of Bcl2/, Bax, cleaved‐caspase3, cleaved‐caspase9, and cleaved‐PARP expression in the indicated cells exposed to cisplatin (100μg/ml). GAPDH was used as a loading control. (D) Analysis of mRNA level (left) and correlation (right) of COMMD10 with BAX, BCL2, CASP3, and CASP9 in 10 freshly collected human HCC samples. The data are shown from a single representative experiment of three repeats. Each bar represents the mean ± SD; *p < 0.05; **p < 0.01; ***p < 0.001