FIGURE 4.

SFN activate cardiac Nrf2 partially through inhibiting CPG islands methylation in the Nrf2 promoter. A, Bisulfite genomic sequencing was applied to assess the CpGs methylation status in the promoter of Nrf2 gene. Black dots and open circles presented methylated CpGs and non‐methylated CpGs, respectively. B, The expression of DNMT3a and 3b was detected by western blot. C, The DNMT activity kit was used to examine the total activity of DNMT. Data were presented as the mean ± SD (n = 5‐7). *P < .05 vs control; ^# P < .05 vs Ang II