Table 1.

Osmolality and pH of WLE and WLD in Fischer’s medium

	Cell culture	pH	Osmolalitya
Medium	Fischer’s mediumb	7.31	309 ± 0.4
	F5pc	7.56	305 ± 1.7
WLE	3 mg ml−1 −pH	6.69	319 ± 3.9
	+pH	7.26	317 ± 0.5
	4 mg ml−1 −pH	6.42	321 ± 0.5
	+pH	7.22	325 ± 0.5
	5 mg ml−1 −pH	6.23	330 ± 5.4
	+pH	7.14	333 ± 5.3
WLD	3 mg ml−1 −pH	6.46	320 ± 0.8
	+pH	6.94	321 ± 1.7
	4 mg ml−1 −pH	6.26	324 ± 3.1
	+pH	7.51	330 ± 0.9
	5 mg ml−1 −pH	5.96	327 ± 0.5
	+pH	7.21	330 ± 0.5
	6 mg ml−1 −pH	5.77	331 ± 0.8
	+pH	7.29	343 ± 0.5
	7 mg ml−1 −pH	5.58	338 ± 0.9
	+pH	7.09	347 ± 0.9
	8 mg ml−1 −pH	5.45	341 ± 1.3
	+pH	7.14	349 ± 2.7

“−/+ pH” means the cell culture without or with pH adjustment by directly adding 5 M NaOH to the treatment medium.

^aThe osmolality was averaged based on three measurements and expressed in terms of mOsm kg⁻¹ of H₂O.

^bThe manufacture data sheet for Fischer’s medium indicates pH of 7.2 ± 0.2 and osmolality of 300 ± 5% mOsm kg⁻¹ of H₂O.

^cThe treatment medium (see Materials and Methods).