Table 3.
LOH at different loci along chromosome 11 in large- and small-colony Tk mutants from cells treated with WLE and WLD
| Locus | Positiona | WLE (4.5 mg ml−1) | WLE (5 mg ml−1) | WLD (8 mg ml−1) | Controlb | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| (cM) | (Mbp) | No. of large colonies (%) | No. of small colonies (%) | No. of large colonies (%) | No. of small colonies (%) | No. of large colonies (%) | No. of small colonies (%) | No. of large colonies (%) | No. of small colonies (%) | |
| D11Mit74 | 3.52 | 5.3 | 12 (25) | 4 (8) | 8 (17) | 4 (8) | 8 (17) | 3 (7) | 5 (14) | 9 (27) |
| D11Mit20 | 27.23 | 44.7 | 13 (27) | 5 (10) | 15 (31) | 5 (11) | 10 (21) | 5 (11) | 5 (14) | 13 (39) |
| D11Mit36 | 51.23 | 83.8 | 20 (42) | 7 (15) | 23 (48) | 7 (15) | 12 (25) | 8 (18) | 11 (31) | 16 (49) |
| D11Mit42 | 78.70 | 113.1 | 37 (77) | 32 (67) | 36 (75) | 38 (81) | 31 (65) | 39 (87) | 12 (33) | 29 (88) |
| Tk | 82.96 | 117.8 | 44 (92) | 48 (100) | 43 (90) | 47 (100) | 37 (77) | 45 (100) | 15 (42) | 32 (97) |
| Non-LOHc | 4 (8) | 0 (0) | 5 (10) | 0 (0) | 11 (23) | 0 (0) | 21 (58) | 1 (3) | ||
| Total | 48 | 48 | 48 | 47 | 48 | 45 | 36 | 33 | ||
a
Locus position in centiMorgan (cM) or megabase pairs (Mbp) is the distance to the top of chromosome 11.
b
The data for the negative control are from our previous work.28
c
Retain heterozygosity.