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. 2020 May 21;106(5):1433–1442. doi: 10.3324/haematol.2019.226852

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Figure 5. — Additive effect of 2'-MOE-SSO in homozygous IVS2-745 patient cells (P5). (A) Electrophoresis of polymerase chain reaction (PCR) products of cDNA obtained from erythroblasts from specimen P5. Wild-type (WT) and IVS2-745 β-globin (HBB) amplified products are indicated in untreated and treated specimens at the 50 mM and 100 mM doses. (B) Quatitative PCR analyses of correctly spliced WT HBB mRNA. Values in y-axes indicate HBB expression normalized to the housekeeping glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene expression and red-cell specific glycophorin A gene expression. (C) Percentage of hemoglobin A (HbA) in scramble-treated control (S) and 2'-MOE-SSO treated specimens (91-92-93) at same dose (n=3). All statistical comparisons (ANOVA/Kruskal-Wallis) are tested against scramble control. **P<0.01; ***P<0.001; ****P<0.0001.