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. 2021 Feb 11;106(5):1443–1456. doi: 10.3324/haematol.2020.264523

Figure 1.

Figure 1.

Arsenic and interferon-target adult T-cell leukemia/lymphoma cells and their innate immunity to abrogate adult T-cell leukemic-initiating cell activity. (A) SCID mice were injected with 106 cells, derived from the tumoral spleen of tax transgenic mice that developed adult T-cell leukemia/lymphoma (ATL), treated with arsenic trioxide (AS) and interferon-alpha (IFN) from day 18, for 3 days, then sacrificed. One million unsorted or sorted CD25+high or CD25+low spleen-derived cells from primary recipients were injected into secondary SCID mice. Survival of untreated secondary recipients injected with unsorted spleen cells from untreated (control) and AS/IFN-treated primary mice are shown in black and red, respectively (n=10 per condition). Survival of untreated secondary recipients injected with CD25+high or CD25+low cells from untreated primary mice (control) are shown in black crossed and dashed histograms, respectively; those from AS/IFNα-treated primary mice are shown in red crossed and dashed histograms, respectively (n=4/condition). (B) One million CD25+ cells from control or AS/IFNα-treated primary mice were injected alone (n=5 per condition) or together with 106 spleen-derived CD25- cells from AS/IFN-treated primary mice into secondary SCID mice (n=3/condition). Survival of untreated secondary SCID mice injected with CD25+ cells from control or AS/IFN-treated primary mice are represented in black and red histograms, respectively. Survival of secondary SCID mice injected with CD25+ cells from control mice and CD25- cells from AS/IFN-treated primary mice are represented by a dashed red histogram (black contour line). Survival of secondary SCID mice injected with CD25+ cells and CD25- cells from AS/IFN-treated primary mice are represented by a dashed red histogram (red contour line). (C) Survival of untreated secondary SCID (red histograms) and NOG SCID (blue histograms) mice injected with 106 unsorted spleen cells from control or AS/IFNα-treated primary SCID mice (n=7/condition). The t-test was performed to validate significance. *P≤0.05, **P≤0.01, and ***P≤0.001. P-values ≤0.05 were considered statistically significant.