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. 2020 Apr 2;106(5):1321–1329. doi: 10.3324/haematol.2019.233478

Figure 2.

Figure 2.

The formation and clearance of actin-vitamin D binding protein (VDBP) complexes after hematopoietic stem cell transplant (HSCT) and the impact of VDBP genotype on modulation of VDBP levels after HSCT. (A) Western blot probed for VDBP showing serum collected pre-transplant, and at days 0, 7, and 14 after transplant from two HSCT recipients. A lower molecular weight band corresponding to VDBP (unbound to filamentous actin) is seen in all samples including pre-transplant serum (baseline [BL]). However, in post-transplantation serum a larger slower migrating band was also observed corresponding to VDBP bound to actin collected on days 0, 7 and 14, demonstrating the presence of circulating actin-VDBP complex in samples from one patient, unique patient number (UPN) 198. Similar increase in levels of circulating actin-VDBP complex is not seen in patient UPN 436, illustrating inter-individual variability. BL: sample collected before the start of transplant. (B) Western blot probed for VDBP showing clearance of increased actin-VDBP complexes from the circulation, with complexes largely cleared by days 21-28 after HSCT in two different transplant recipients (UPN 324 and UPN 336). (C) VDBP levels according to genotype, measured at BL, days 0, 7, 14, 30 and 100 after HSCT, showing decreased levels at day 14 in all genotypes.