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. 2020 Apr 23;106(5):1354–1367. doi: 10.3324/haematol.2019.228064

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Figure 7. — In situ hybridization analyses on embryonic day (E) 9.0, E9.5 and E10.5 wild-type embryos. (A) Whole mount in situ hybridization analysis of IL-3R, Sox17 and Gata2 mRNA in E9.0, E9.5 and E10.5 embryos. IL-3R was expressed in the aorta-gonad-mesonephros (AGM) region (see arrowhead), the tailbud mesenchyme, the limbs, the branchial arches and the somites from E9.0 until E10.5. Sox17 mRNA was expressed in these tissues at all analysed stages. Gata2 transcripts were found in the caudal head mesenchyme from E9.0 until E9.5 and additionally in the otic vesicle, the midbrain and the AGM region at E10.5. (B) RNA in situ hybridization analysis of transverse sections of the E9.5 embryo.