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Identification of SUMO ligases as Sgo1 regulators. (A) Overview of multicopy suppressor screen to identify Sgo1 antagonists. (B) Deletion of CDC55 partially alleviates the metaphase delay phenotype of the siz1Δ siz2Δ mutant. Mitotic time course analysis was performed as described in Fig. 1 D for the following strains: wild type (AMy8467), cdc55Δ (AMy8779), siz1Δ siz2Δ (AMy8452), and siz1Δ siz2Δ cdc55Δ (AMy8637).
