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. 2021 Apr 29;218(6):e20200560. doi: 10.1084/jem.20200560

Figure 6.

Figure 6.

Cebpa knockout counteracts chronic IL-1β–driven transcriptional programs. (A–C) GSEA using “Chambers all myeloid,” “hallmark mTORC1 signaling,” and “Giladi cell cycle” gene sets. (A) Schematic and enrichment plots for pairwise comparison between Cebpa+/+ MPP3 isolated from chronic IL-1β–treated versus vehicle-treated mice (“chronic IL-1β effect”). (B) Schematic and enrichment plots for pairwise comparison between CebpaΔ/Δ and Cebpa+/+ MPP3s isolated from chronic IL-1β–treated mice (“CebpaΔ/Δ modification of chronic IL-1β effect”). (C) Heatmaps for leading edge genes from B (genes for heatmap are listed in Table S1). (D) Experimental design for cell cycle analysis (n = 5 per group). (E) Representative flow cytometry plots for Ki67/DAPI cell cycle analysis. (F) Graph of frequency of MPP3 in G0, G1, S/G2/M. FDR, false discovery rate; NES, normalized enrichment score. In D–F, cell cycle data are from one experiment with n = 5 mice per group, presented as mean ± SD, and analyzed by two-way ANOVA with Tukey’s multiple comparisons test. *, P < 0.05; **, P < 0.01.