Table 2.
Corrected instrument configuration corresponding to online Table 1
| Laser Wavelength (nm) | Laser Power (mW) | Laser Type | Detector | Spectral Range for Detector (nm) | Dichroic LP Mirror (nm) | Band Pass (nm) | Example Fluorochrome |
|---|---|---|---|---|---|---|---|
| 637 (Red) | 200 | DPSS | R780 | 750–810 | 750 | 780/60 | APC‐Cy7 |
| R710 | 723–753 | 685 | 730/45 | Alexa700 | |||
| R660 | 655–685 | 630 | 670/30 | APC | |||
| 532 (Green) | 200 | DPSS | G780 | 750–810 | 750 | 780/60 | PE‐Cy7 |
| G710 | 685–735 | 690 | 710/50 | PE‐Cy5.5 | |||
| G660 | 640–680 | 635 | 660/40 | PE‐Cy5 | |||
| G610 | 600–620 | 600 | 610/20 | PE‐Dazzle594 | |||
| G575 | 563–588 | None | 575/25 | PE | |||
| 488 (Blue) | 200 | DPSS | B780 | 760–800 | 740 | 780/40 | BB790 |
| B710 | 685–735 | 690 | 710/50 | BB700 | |||
| B660 | 640–680 | 635 | 660/40 | BB660 | |||
| B610 | 600–620 | 600 | 610/20 | BB630 | |||
| B515 | 505–525 | 505 | 515/20 | FITC | |||
| 405 (Violet) | 200 | DPSS | V780 | 754–816 | 770 | 785/62 | BV785 |
| V750 | 735–765 | 735 | 750/30 | BV750 | |||
| V710 | 690–730 | 685 | 710/40 | BV711 | |||
| V655 | 650–670 | 630 | 660/20 | BV650 | |||
| V682.510110 | 595–615 | 580 | 605/20 | BV605 | |||
| V570 | 563–588 | 550 | 575/25 | BV570 | |||
| V510 | 505–525 | 505 | 515/20 | BV510 | |||
| V450 | 430–470 | None | 450/40 | V450 | |||
| 355 (Ultraviolet) | 60 | DPSS | U780 | 754–816 | 755 | 785/62 | BUV805 |
| U730 | 723–758 | 710 | 740/35 | BUV737 | |||
| U660 | 640–680 | 635 | 660/40 | BUV661 | |||
| U570 | 550–590 | 550 | 570/40 | BUV563 | |||
| U500 | 500–530 | 470 | 515/30 | BUV495 | |||
| U450 | 425–475 | 410 | 450/50 | Viability | |||
| U395 | 365–393 | None | 379/28 | BUV395 |