Figure 2.

DEP‐1 knockdown does not affect proliferation but reduces sensitivity to drug‐induced apoptosis. A. KT21 and SF3061 cells stably transfected with DEP‐1‐targeting or control shRNA expression plasmids were seeded in 96‐well plates in growth medium containing 10% FCS. After the indicated times, MTT reagent was added for 4 h, the cells were lysed and OD 570 nm was measured. The mean of seven replicates ± SD of one representative experiment out of three with consistent results is shown. B. KT21 cells stably transfected with DEP‐1‐targeting or control shRNA expression plasmids were left untreated or were treated for 24 h with staurosporine in the indicated concentrations, and then subjected to a caspase‐3 activity assay to assess apoptosis induction (mean values ± SD, n = 10, *P < 0.05 by t‐test).