Table 2.
Antibodies used for immunohistochemistry. Abbreviations: AQP4 = aquaporin‐4; GFAP = glial fibrillary acidic protein; N.D. = not done.
| Antibody | Type | Dilution | Antigen retrieval | Source |
|---|---|---|---|---|
| Aquaporin‐4 | ||||
| AQP4 | rabbit polyclonal | 1:500 | N.D. | Santa Cruz Biotechnology, California, USA |
| Complement | ||||
| C3d | rabbit polyclonal | 1:1000 | Autoclave/10 mM citrate buffer | DakoCytomation, Glostrup, Denmark |
| C9neo | mouse monoclonal | 1:1000 | Autoclave/10 mM citrate buffer | Abcam plc, Cambridge, UK |
| Macrophage/microglia | ||||
| CD68 | mouse monoclonal | 1:200 | Autoclave/10 mM citrate buffer | DakoCytomation, Glostrup, Denmark |
| Astrocyte | ||||
| GFAP | rabbit polyclonal | 1:1000 | N.D. | DakoCytomation, Glostrup, Denmark |
| Immunoglobulin | ||||
| IgG | rabbit polyclonal | 1:10 000 | Autoclave/10 mM citrate buffer | DakoCytomation, Glostrup, Denmark |
| IgM | rabbit polyclonal | 1:10 000 | Autoclave/10 mM citrate buffer | DakoCytomation, Glostrup, Denmark |
| Lymphocyte | ||||
| CD45RO | mouse monoclonal | 1:200 | Autoclave/10 mM citrate buffer | DakoCytomation, Glostrup, Denmark |
| CD20 | mouse monoclonal | 1:200 | Autoclave/10 mM citrate buffer | DakoCytomation, Glostrup, Denmark |