Summary of findings 2. Preimplantation genetic testing for aneuploidies with the use of fluorescence in situ hybridisation (FISH) for the genetic analysis in in vitro fertilisation.
| Preimplantation genetic testing for aneuploidies with the use of fluorescence in situ hybridisation (FISH) for the genetic analysis in in vitro fertilisation | |||||
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Patient or population: couples with an in vitro fertilisation indication
Settings: fertility clinics
Intervention: PGT‐A with the use of FISH for the genetic analysis Comparison: no PGT‐A | |||||
| Outcomes per woman | Illustrative comparative risks* (95% CI) | Relative effect (95% CI) | No. of participants (studies) | Quality of the evidence (GRADE) | |
| Assumed risk | Corresponding risk | ||||
| Control | PGT‐A with the use of FISH for the genetic analysis | ||||
| Cumulative live birth | 287 per 1000 | 192 per 1000 (124 to 289) | OR 0.59 (0.35 to 1.01) | 408 (1 RCT) | ⊕⊕⊝⊝ lowa |
| Live birth rate after the first embryo transfer | 307 per 1000 | 215 per 1000 (160 to 287) | OR 0.62 (0.43 to 0.91) | 1680 (10 RCTs) | ⊕⊕⊕⊝ moderateb |
| Miscarriage | 105 per 1000 | 108 per 1000 (81 to 143) | OR 1.03 (0.75 to 1.41) | 1680 (10 RCTs) | ⊕⊕⊕⊝ moderatec |
| Ongoing pregnancy | 274 per 1000 | 204 per 1000 (161 to 253) | OR 0.68 (0.51 to 0.90) | 1121 (5 RCTs) | ⊕⊕⊝⊝ lowb,c |
| Clinical pregnancy | 333 per 1000 | 219 per 1000 (174 to 267) | OR 0.56 (0.42 to 0.73) | 1131 (5 RCTs) | ⊕⊕⊕⊝ moderatec |
| *The basis for the assumed risk (e.g. the median control group risk across studies) is provided in footnotes. The corresponding risk (and its 95% confidence interval) is based on the assumed risk in the comparison group and the relative effect of the intervention (and its 95% CI). CI: confidence interval; OR: odds ratio; PGT‐A: preimplantation genetic testing for aneuploidies; RCT: randomised controlled trial | |||||
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GRADE Working Group grades of evidence
High quality: further research is very unlikely to change our confidence in the estimate of effect. Moderate quality: further research is likely to have an important impact on our confidence in the estimate of effect and may change the estimate. Low quality: further research is very likely to have an important impact on our confidence in the estimate of effect and is likely to change the estimate. Very low quality: we are very uncertain about the estimate. | |||||
aDowngraded two levels for imprecision: results based on one study, small number of events. bDowngraded one level for inconsistency: I² > 50% in results across studies. cDowngraded one level for imprecision: the CI for most studies crosses the line of no effect, or small number of events.