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. 2010 Dec 3;21(1):1–15. doi: 10.1111/j.1750-3639.2010.00417.x

Figure 1.

Figure 1

Net synapse loss on hypoglossal motoneurons (HMNs) from early‐symptomatic hSOD1G93A mice. A. B. Synaptophysin‐immunoreactive (syn‐ir) puncta around FluoroGold (FG; Molecular Probes, Eugene, OR, USA)‐backlabeled HMNs from non‐transgenic wild‐type (WT) littermates (A) and transgenic (B) control mice at 90 days postnatal (P90). Secondary antibody was labeled with cyanine 5. C, D. Electron microscopy photomicrographs showing a HMN (C) and a hypoglossal interneuron (D) obtained from a control mouse. Outlined arrow signals the nuclear invagination characteristic of interneurons from the hypoglossal nucleus. E, F. Segments of plasma membrane of HMNs obtained from the indicated mice at P90. Solid arrows point to synaptic boutons attached to the plasma membrane. Note the low frequency of boutons attached to the HMN from the hSOD1G93A mouse. G. Table showing the average number of Syn‐ir puncta (confocal microscopy analysis) and synaptic boutons (electron microscopy analysis) relative to 100 µm of membrane perimeter of HMNs from 3‐month‐old mice with the indicated genotypes. The number of analyzed HMNs from three different mice per genotype is indicated in parentheses. **P < 0.001, two‐way analysis of variance, post hoc Tukey's test. Scale bars, A–D, 10 µm; E, F, 1 µm.