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. 2010 Nov 3;21(3):249–262. doi: 10.1111/j.1750-3639.2010.00443.x

Figure 5.

Figure 5

Effects of siRNA targeting HIF‐1αon Nix and VEGF expression. A. and B. Time course of HIF‐1α expression in primary neurons after been transfected with siRNA into cultured cortical neurons 30 h before (A) or 12 h after (B) being subjected to OGD. HIF‐1α expression was detected by immunoblotting with anti‐HIF‐1α antibodies, using actin as an internal control. C. and D. Primary neurons were transfected with siRNA either 30 h before or 12 h after being subjected to OGD. Total lysates were immunoblotted with either anti‐Nix antibody or anti‐VEGF antibodies, using actin as an internal control. E. The viability of the cultured cortical neurons was assessed by using the MTS assay. All experiments were performed in triplicate, and the results were expressed as a percentage of the levels in the naive‐controls. Statistical analysis was carried out using the one‐way ANOVA with appropriate post hoc tests; *P < 0.05; **P < 0.01 vs. the naive‐control group. Abbreviations: siRNA = Small interfering RNA; HIF‐1α = hypoxia‐inducible factor‐1‐alpha; Nix = Nip‐like protein X; VEGF = vascular endothelial growth factor; OGD = oxygen–glucose deprivation; MTS = methanethiosulfonate; Luc = luciferase.