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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: J Exp Zool B Mol Dev Evol. 2020 Aug 30;334(6):325–338. doi: 10.1002/jez.b.22998

Figure 6: Dorsoventral expression of Tbx5 during gas bladder development in bowfin.

Figure 6:

A) Boxplot of Tbx5 expression at pre-budding, budding, and outgrowth stages of gasbladder development. Tbx5 expression in the dorsal and ventral mesoderm is equally high during pre-budding (pink and light green), but as development proceeds through budding and outgrowth, dorsal expression increases while ventral expression decreases. B) RNAscope in situ hybridization of Tbx5 during gas bladder development in bowfin compared with lung development in bichir. During gas bladder outgrowth (stage 27) in bowfin, Tbx5 is expressed most strongly in the dorsal mesoderm surrounding the gas bladder (top panels). During lung outgrowth (stage 35) in bichir, Tbx5 is expressed in the ventral mesoderm surrounding the developing lungs (bottom panels). The left side of panels show Tbx5 expression in bright red overlaid with DAPI (bright blue), which stains nuclei, and the right panels show Tbx5 expression alone in bright red. Abbreviations: fg, foregut; gb, gas bladder; lL, left lung; rL, right lung.