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. 2021 May 4;14:113. doi: 10.1186/s13068-021-01962-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Heterologous Pta activity in Y. lipolytica. PTA genes from seven different

source organisms codon-optimized to S. cerevisiae (GeneArt) were expressed in YB-392 under the control of the Y. lipolytica EXP1 promoter using linear integrating expression cassettes. Cell-free extracts (CFE) from four transformants per test gene were analyzed for Pta activity using a DTNB assay. Data are presented as fold change of the measured specific activity over the averaged specific activity of the parent strain YB-392 (dashed line), which is included as control