Figure 5.

IL-17A upregulates FABP4 and activates the NLRP3 inflammasome

(A) The protein levels of FABP4 and NLRP3 inflammasome components were determined by western blot. (B) The protein levels of NF-κB signaling components were detected by western blot. (C) The protein levels of ERK and p38 MAPK signaling components were determined by western blot. (D) The protein levels of ER stress-related proteins were examined by western blot. β-actin served as a loading control. (E) IF staining of NLRP3 (green) and ASC (red). Nuclei was visualized by DAPI (blue). (F) The protein levels of FABP4 and NLRP3 inflammasome components in placental tissues were determined by western blot. (G) The immunoreactivities of FABP4 in placental tissues were monitored by IHC analysis. Data are expressed as representative images or the mean ± SD of n = 3 experiments.