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. Author manuscript; available in PMC: 2021 May 4.
Published in final edited form as: Methods Mol Biol. 2021;2251:225–236. doi: 10.1007/978-1-0716-1142-5_17

Fig. 2.

Fig. 2

Phospholipase C activity of PLC-γ1 (D1165H) in lipid vesicles. (a) XY-69 (0.5 μM) was embedded into lipid vesicles containing PE (200 μM) and PIP2 (20 μM) and added to PLC-γ1 (D1165H) with indicated concentration to initiate the hydrolysis. The reaction progression at 20 °C was monitored continuously for 60 min by fluorescence (λex/λem = 485/520 nm). The final assay buffer has a pH of 7.4 and free Ca2+ concentration at 390 nM. (b) The concentration of PE and PIP2 in lipid vesicles was 192 and 48 μM, respectively. The experiment was run similarly as described in (a)