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. 2010 Apr 16;20(6):1021–1032. doi: 10.1111/j.1750-3639.2010.00405.x

Figure 3.

Figure 3

A. Genomic organization of the human p53‐depdendent apoptosis modulator (PDAM) gene. Open box represents exon and dark box indicates predicted coding region (NM_207306). The early cryptic stop codon (underlined) positions upstream of the translational start codon (bold) is also shown. The 36‐bp repeat region is represented by dotted box and the AluSg sequence is indicated by hatched box. B. Tissue distribution of PDAM transcripts. Blot containing polyA‐RNA of various human tissues was probed with the radioactively labeled PDAM‐predicted coding cDNA. At least nine isoforms (dark circle) are discernable. The 3.7‐kb isoform appears to be present in all tissues examined. C. Coupled in vitro transcription–translation assay shows no translatable proteins from the full‐length PDAM cDNA [pBluescript (pBS)‐PDAM]. A product of ∼27 kDa is observed with pBS green fluorescent protein (pBS‐GFP). As controls, cDNAs cloned in reverse orientation (pBS‐PDAMr and pBS‐GFPr) yield no detectable products.