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. 2010 May 13;20(6):1042–1054. doi: 10.1111/j.1750-3639.2010.00410.x

Figure 6.

Figure 6

Observation of TH expression after rno‐let‐7d transfection. A. In PC12L cells, TH expression was detected by Western blot 72 h after transient transfection with rno‐let‐7d precursor. B. The band density in A was analyzed by the software Image J (Wayne Rasband, National Institute of Health, USA). Data were shown as an average of relative density with standard errors, where n = 3. (**P < 0.01). C. TH mRNA levels were detected by RT‐PCR in PC12L cells transfected with rno‐let‐7d precursor. D. Relative luciferase activity in CBRH‐7919 and PC12L cells was shown 48 h after co‐transfection with rno‐let‐7d precursor and report plasmids. The results were representative of three independent experiments. Abbreviations: PC12L = low differentiated pheochromocytoma cells; RA = retinoic acid; RT–PCR = reverse transcriptase–polymerase chain reaction; TH = tyrosine hydroxylase.