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. 2010 Apr 14;20(6):1010–1020. doi: 10.1111/j.1750-3639.2010.00400.x

Figure 3.

Figure 3

Exemplary images of gel electrophoresis of DNA amplified using PCR with primers for DNA fragments of 100 (lowest band), 200, 300, 400, 600 base pairs (bp) of two brain tumor cases. (A) Representative image for the majority of cases showing amplifiable DNA up to a fragment length of 600 bp in FFPE, RCLPE and frozen specimens (Case 7, Table 3). (B) In this case with prolonged fixation duration of 6 days for RCLPE and 3 days for FFPE (Case 12, Table 3), DNA extracted from frozen (Fr) and RCLPE (R) specimens is amplifiable up to a fragment length of 600 base pairs (bp), although the RCLPE 600 bp band is relatively weak. DNA extracted from the FFPE (F) tissue sample is amplifiable only up to a fragment length of 400 bp. The 300 bp and 400 bp bands are markedly weaker in the FFPE specimen as compared to the RCLPE specimen. Thus, there is evidence of significant DNA degradation in FFPE material.