Table 2.

Summary of antibodies and staining protocols used in this study. Abbreviations: FFPE = formalin‐fixed and paraffin‐embedded; RCLPE = RCL2‐fixed and paraffin‐embedded.

Visualized antigen	Supplier (antibody, clonality)	Fixation type	Pretreatment (Supplier)	Antibody dilution	Antibody incubation time
GFAP	Dako, Glostrup, Denmark (Anti‐GFAP, pc)	FFPE	5 min Proteinase K (Dako)	1:3000	30 min
		RCLPE	none	1:3000	30 min
S‐100 protein	Dako, Glostrup, Denmark (Anti‐S100 protein, pc)	FFPE	none	1:500	30 min
		RCLPE	none	1:500	30 min
Vimentin	Dako, Glostrup, Denmark (V9, mc)	FFPE	none	1:50	30 min
		RCLPE	none	1:400	30 min
EMA	Dako, Glostrup, Denmark (E29, mc)	FFPE	FLEX TRS low (Dako)	1:100	30 min
		RCLPE	none	1:100	30 min
Synaptophysin	Dako, Glostrup, Denmark (SY38, mc)	FFPE	FLEX TRS low (Dako)	1:200	30 min
		RCLPE	FLEX TRS low (Dako)	1:200	30 min
NeuN	Chemicon, Tomecula, California, USA (A60, mc)	FFPE	FLEX TRS low (Dako)	1:1000	30 min
		RCLPE	FLEX TRS low (Dako)	1:1000	30 min
Map‐2	Chemicon, Tomecula, California, USA (AP20, mc)	FFPE	FLEX TRS low (Dako)	1:8000	30 min
		RCLPE	FLEX TRS low (Dako)	1:8000	30 min
Phosphorylated neurofilament H and M	Sternberger Monoclonals, Lutherville, Maryland, USA (SMI31, mc)	FFPE	FLEX TRS low (Dako)	1:50.000	30 min
		RCLPE	FLEX TRS low (Dako)	1:50.000	30 min
Nonphosphorylated neurofilament H	Sternberger Monoclonals, Lutherville, MD, USA (SMI32, mc)	FFPE	FLEX TRS low (Dako)	1:200	30 min
		RCLPE	FLEX TRS low (Dako)	1:1000	30 min
NSE	Dako, Glostrup, Denmark (BBS/NC/VI‐H14, mc)	FFPE	FLEX TRS low (Dako)	1:1000	30 min
		RCLPE	FLEX TRS low (Dako)	1:1000	30 min
CK	BMA Biomedicals (Lu‐5, mc)	FFPE	5 min Proteinase K (Dako)	1:100	30 min
		RCLPE	none	1:10	30 min
Ki‐67	Dako, Glostrup, Denmark (MIB1, mc)	FFPE	FLEX TRS low (Dako)	1:200	30 min
		RCLPE	FLEX TRS high (Dako)	1:200	30 min